赖氨匹林对小鼠移植乳腺癌的生长抑制和诱导凋亡作用

Growth inhibition and apoptosis inducted by aspisol on transplanted breast cancer of mice

目的:观察不同浓度的赖氨匹林对C3H小鼠移植性乳腺癌的抑制作用、肿瘤细胞凋亡和造血系统及肝肾功能的影响,为赖氨匹林抗肿瘤作用的临床应用研究提供实验佐证。方法:用细胞悬液法将肿瘤细胞接种于C3H小鼠前肢腋窝皮下,接种后将小鼠随机分成aspisol大、中、小剂量组和生理盐水(NS)组及氟尿嘧啶(5-Fu)组,于接种后第2天分别腹腔注射相应药物,1次/天,连续给药28天。第30天取血,处死小鼠,称瘤体重量,计算抑瘤率;观察小鼠对造血系统、肝肾功能的变化;观察肿瘤组织病理变化;TUNEL法检测赖氨匹林对肿瘤细胞凋亡的影响。结果:赖氨匹林对小鼠乳腺癌的生长具有显著的抑制作用(P<0.01),苏木精-伊红(HE)染色组织病理学检查显示:赖氨匹林各剂量组肿瘤组织有不同程度的坏死。各治疗组肿瘤细胞均呈现明显凋亡形态改变,在一定的用药时间和用药范围内赖氨匹林对小鼠的造血系统及肝、肾功能无明显影响。结论:赖氨匹林对C3H小鼠移植乳腺癌的生长具有抑制作用,能诱导其凋亡,但对其造血系统及肝、肾功能无明显影响。

Objective:To observe the antineoplastic action of aspisol on transplanted tumors of C3H mice and the influence of aspisol on hematopoietic organs,hepatic function and renal function of mice.Tumor cell apoptosis had also been determined for providing experiment bases of aspisol in breast cancer therapy.Methods:The suspension of cells was injected subcutaneously into forelimb axillas of C3H mice.From the next day,the explanted animals were received different concentration of aspisol by intraperitoneally once a day for 28 days.The reference group was given 5-Fu and control group was given normal saline.At 30th day after treatment,the mice were sacrificed,tumor weight were examined,haemocyte number were calculated,hepatic function and renal function were detected.The histopathological change were observed microscopically,TUNEL assay in situ were performed for determine the apoptosis.Results:aspisol can inhibit the growth of tumor significantly,the tumor volume were dramatically reduced(P<0.01).Histological examination results showed apparent necrosis in aspisol group.Typical apoptotic morphological changes were seen in all the treated groups.aspisol didn't affect hematopoietic organs,hepatic function and renal function of the tumor bearing animals at certain time or dosage.Conclusions:aspisol has significantly antitumor effects on C3H mice transplantable breast cancer,can induce tumor cell apoptosis,without obvious influence on hematopoietic system,hepatic function and renal function.