高危型人乳头状瘤病毒在子宫颈癌组织和外周血表达的意义

Expression of high risk type human papillomavirus in the tissues and peripheral blood of cervical carcinomas

目的:分别检测子宫颈癌组织和外周血标本中人乳头状瘤病毒(HPV)(16型,18型),探讨HPV感染与子宫颈癌的关联性以及在肿瘤微转移中的表达.方法:采用荧光定量PCR(FQ-PCR)和逆转录PCR(RT-PCR)技术检测24份子宫颈癌组织及其治疗前后48份外周血标本,慢性子宫颈炎组织及其外周血标本各20份.结果:24份子宫颈癌组织(Ⅰ～Ⅱb期)中HPV DNA 16型阳性率为66.7%,HPV DNA 18型阳性率为29.2%,两型均阳性的阳性率为8.3%;48份外周血标本HPV mRNA 16型阳性率为2.1%;20份慢性子宫颈炎组织HPV DNA 16型阳性率为15.0%,HPV18型为阴性,其外周血均为阴性.子宫颈癌HPV DNA 16型组与慢性子宫颈炎HPV DNA 16型组比较差异有统计学意义(P＜0.005～P＜0.05).结论:高危型HPV感染与子宫颈癌发生高度相关.外周血HPV检出率低,临床价值有待进一步研究.

Objective:To detect expression of human papillomavirus(HPV) type 16 and 18 in carcinoma tissues and peripheral blood of patients with cervical carcinomas,and to explore the relationship between HPV infection and cervical carcinomas,as well as its expression in tumour micrometastase.Methods:Real-time fluorescence quantitative PCR(FQ-PCR) and reverse transcription polymerase chain reaction(RT-PCR) were used to detect HPV in 24 cases of tissues and 48 samples of peripheral blood of cervical carcinomas,and 20 cases of tissues and 20 smples of peripheral blood of chronic cervicitis.Results:In the 24 tissues of cervical carcinomas(Ⅰ-Ⅱb),the positive rate of HPV DNA type 16 was 66.7%;the positive rate of HPV DNA type 18 was 29.2%;the positive rate of both positive HPV DNA 16 and HPV DNA 18 was 8.0%.In the 48 samples of peripheral blood cervical carcinomas,the positive rate of HPV mRNA type 16 was 2.1%.In the 20 chronic cervicitis tissues,the positive rate of HPV DNA type 16 was 15.0% and HPV DNA type 18 was negative.Both HPV type 16 and 18 of peripheral blood were negative.The difference was markedly significant between cervical carcinomas and chronic cervicitis(P<0.005-P<0.05).Conclusions:The quantitation of HPV is highly associated with cervical carcinomas.Detection of HPV mRNA in the peripheral blood of cervical carcinomas is low,and its clinical significance needs further evaluation.