胃癌患者MMP-2、CEA的表达及其临床意义

Expression and significance of MMP-2 and CEA in patients with gastric carcinoma

目的:探讨胃癌患者基质金属蛋白酶2(MMP-2)、癌胚抗原(CEA)的表达及其临床意义 . 方法:采用免疫组化S-P 法测定胃癌患者活检或手术标本中的MMP-2表达,采用免疫放射分析法(IRMA)检测空腹血清CEA 水平,并分析其与分化程度、TNM分期、淋巴结、肝、胰转移和腹膜、脉管受侵犯的关系.结果:CEA血清水平与淋巴结、肝脏、腹腔转移均有一定关系(P＜0.01～P＜0.005),而与胃癌分化程度、胰腺转移、脉管转移、临床分期均无关系(P＞0.05).MMP-2表达强度与淋巴结、肝脏、胰腺、脉管和腹腔转移与否差异均有统计学意义(P＜0.005),与胃癌分化程度、临床分期均无明显关系(P＞0.05).结论:MMP-2、CEA在胃癌的发生和侵袭转移中可能起着重要作用,联合检测MMP-2、CEA的表达有望成为判断胃癌病变发展、预测肿瘤转移潜能的客观指标.

Objective:To investigate the expression and clinical significance of matrix metalloproteinase-2(MMP-2) and carcino embryonic antigen(CEA) in patients with gastric carcinoma.Methods:Bioptic and resectic specimens were examined by S-P immunohistochemical method for MMP-2;the content of serum CEA was detected by immunoradicmetricassay,and their relations with the differentiation degree of gastric carcinoma,TNM stage,lymph node metastasis,liver metastasis,pancreas metastasis,degree of peritoneum and vascular system invovlement were analyzed.Results:Expression of CEA was positively related to the lymph node metastasis,liver metastasis and peritoneum metastasis(P<0.01-P<0.005),but was not related to the differentiation of gastric carcinoma,pancreas metastasis,vascular system metastasis or TNM stage(P>0.05).Expression of MMP-2 protein was positively related to the lymph node metastasis,liver metastasis,pancreas metastasis,vascular system metastasis and peritoneum metastasis(P< 0.005),but was not related to the differentiation degree of gastric carcinoma or TNM stage(P>0.05).Conclusions:CEA and MMP-2 may play an important role in the formation,invasion and metastasis of gastric carcinoma.Expression of them might serve as an objective indicator for estimating tumor pregress and predicting the metastasis of cancer cells.