摘要
目的建立检测SC的ELISA方法。方法采用交叉方阵滴定法,比较3种封闭液、不同稀释度的包被抗体和酶标抗体对检测结果的影响,确定ELISA方法的最佳实验条件。结果包被抗体浓度1∶400,酶标抗体浓度为1∶800,最适封闭条件为0.5%新生胎牛血清。检测240例肝脏病变患者血清和粪便中SC明显升高,与正常对照组比较均有显著性差异(P<0.01)。结论建立的双抗体夹心ELISA方法检测SC简便实用,可用于临床和科研研究。
Objective To develop a new double antibody sandwich ELISA kit for detecting secretory component.Methods 4 kinds of block buffer、the different concentration of anti-SC and streptavidin-HRP-anti-SIgA are used for titration of ELISA test.Results The optinal dilutions of anti-SC was 1∶400,The optinal dilutions of streptavidin-HRP-anti-SIgA was 1∶800,the optional inclusion reagent was 0.5%fetus serum.The levels of SC in the serum and fecal were notably elevated in 240 hepatic diseases compared with the control(P<0.01).Conclusion ELISA method was a simple and convenient and practical.This method may be used in clinical examination and science study.
出处
《中国实验诊断学》
2007年第11期1524-1526,共3页
Chinese Journal of Laboratory Diagnosis
基金
辽宁省教委课题资助项目
编号为05L466
关键词
分泌片
ELISA方法
肝脏病变
secretory component
ELISA method
hepatic diseases
