摘要
We amplified the 340 bp of mitochondrial DMA (mtDNA) by PCR including the recognized sequence of restriction enzyme of SfaN I . After amplification and digestion of SfaN I , two bands of 190 bp and 150 bp appeared in the mtDNA of four normal individuals but only one band of 340 bp appeared in the mtDNA with the mutation of G to A at the site of the nucleotide 11778 because such mutation destroyed the recognized sequence of SfaN I . We studied the mtDNAs of the patients with Leber's hereditary optic neur...
We amplified the 340 bp of mitochondrial DMA (mtDNA) by PCR including the recognized sequence of restriction enzyme of SfaN I . After amplification and digestion of SfaN I , two bands of 190 bp and 150 bp appeared in the mtDNA of four normal individuals but only one band of 340 bp appeared in the mtDNA with the mutation of G to A at the site of the nucleotide 11778 because such mutation destroyed the recognized sequence of SfaN I . We studied the mtDNAs of the patients with Leber's hereditary optic neuropat...
作者
Lishan Zhang, Ying Huang, Fangyuan Li, ShijunWang, Bin Zhu Ziping Zhang, Yi Tong, Jinjuan GaoDepartment of Biology, Nanjing Railway Medical College Nanjing 210009, ChinaDepartment of Opthahalmology, Fujian Medical College Fuzhou 350005, China
出处
《眼科学报》
1994年第3期151-156,共6页
Eye Science
基金
National Natural Sciences Foundation of China
