摘要
本文从四膜虫分离得到了一定量的镱结合膜蛋白並测定了它的摩尔消光系数。由平衡渗透法及Scatchard类型图,测得该镱-膜蛋白络合物解离常数和结合数的平均值分别为(4.2±0.1)×10^(-6)Smol/l和3,4±0.7,即此络合物的表观稳定常数为2.4×10~5l/mol,或IgK_(?)=5.4.对镱结合膜蛋白及无镱结合膜蛋白氨基酸组分的分析表明,二者之间无明显区别。
Yb binding membrane protein of tetrahymena pyriformis was isolated in considerable amount.The average dissociation constant and the average binding site number of the Yb-protein complex were determined as (4.2±0.1)×10^(-6) mol/l and 3.4±0.7 respectively by equilibrium dialysis experiment and concomitant Scatchard-type diagram.Hence, the apparent stability constant of this complex is around 2.4x10^5l/mol, or 1gK=5.4.No apparent difference on the aminoacid composition between Yb binding protein and correspond...
出处
《同位素》
CAS
北大核心
1990年第3期143-148,142,共7页
Journal of Isotopes
基金
国家自然科学基金
关键词
希土结合膜蛋白
镱-膜蛋白络合物
解离常数
结合数
rare-earth binding protein
Yb-protein complex
dissociation constant
binding site number
