混合制剂中快速计数双歧杆菌鉴别培养基的研究

Study on identifical medium for rapidly enumerate Bifidobacterium in mixture

研制了一种混合制剂中快速计数双歧杆菌鉴别培养基。用添加X-Gal的改良MRS培养基和厌氧胶法对4种标准双歧杆菌(长双歧杆菌、短双歧杆菌、青春双歧杆菌、两歧双歧杆菌)和2种标准乳酸菌(保加利亚乳杆菌、嗜热链球菌)分别单独培养、混合培养,结果在X-Gal培养基上,双歧杆菌单独培养及与乳酸菌混合培养双歧杆菌均为白色菌落或浅蓝色菌落,而乳酸菌为蓝色菌落。结果表明,在X-Gal培养基上双歧杆菌和乳酸菌有明显的鉴别性,可用于双歧杆菌和乳酸菌混合制剂的菌落鉴别计数。

Study on rapidly enumerate bifidobacterium identifical medium in mixture. Four standard Bifidobacterium and two standard Lactobacillus were cultured and mixedly culture separately using modified MRS medium added X Gal. Bifidobacteria was white or light colonies, while Lactobacillus was blue colonies. X Gal medium can be used to enumerate Bifidobacteria and Lactobacillus.