化疗栓塞对肝细胞癌PI、SPF、DI的影响

The Effect of Different Chemoembolization Methods on PI, SPF and DI in Different Histopathological Types of Hepatocellular Carcinoma

目的 研究不同化疗栓塞方法对不同组织学类型的肝细胞癌 (hepatocellularcarcinoma ,HCC)增殖指数 (proliferativeindex ,PI)、S期细胞比率 (S phasefraction ,SPF)、DNA指数 (DNAindex ,DI)的影响及PI、SPF、DI与HCC转移的关系 ,以便进一步研究化疗栓塞对HCC转移潜能的影响。材料与方法 7种不同组织学类型的HCC共 98例 ,单纯手术 5 7例 ,4种化疗栓塞治疗后Ⅱ期手术切除 41例。采用流式细胞术 (flowcytometry ,FCM)检测各标本PI、SPF和DI ,并与转移情况对照。结果 PI和SPF与HCC转移潜能密切相关 (P <0 .0 1,P <0 .0 5 ) ,DI与转移关系不大 (P >0 .0 5 )。PI、SPF和DI存在组织学差异 ,Ⅲ级高于Ⅰ级 ,梁索型、索状腺样型、透明细胞型低于实体型和低 (未 )分化型。化疗栓塞使HCCPI、SPF下降 ,其中多材料联合化疗栓塞组 (C组和D组 )下降最明显 (P <0 .0 5 ) ;单材料化疗栓塞组 (B组 )PI上升最显著 (P <0 .0 5 ) ;重复 3～ 4次化疗栓塞PI、SPF下降显著 (P <0 .0 5 ,P <0 .0 1) ;化疗栓塞后1～ 2个月PI、SPF下降 ,2个月后增高。结论 HCC高转移性与高PI、SPF相关 ,化疗栓塞通过抑制HCC的PI、SPF来抑制其转移潜能 ,多次、多材料联合化疗栓塞且重复治疗间隔

Objective To study the effect of different chemoembolization methods on the proliferative index (PI), S phase fraction (SPF) and DNA index (DI), to discuss the relationship between PI, SPF, DI and metastases, and to make a further understanding of the influence of chemoembolization therapy on the metastatic potentiality of HCC.Materials and Methods Of 98 patients with histologically proved HCC in this study, 57 were treated with surgical resection alone and 41 received second stage surgical resection af...