摘要
目的建立以反相高效液相色谱法同时测定散利痛片中咖啡因、对乙酰氨基酚、异丙基安替比林3组分血药浓度的方法。方法色谱柱为HypersilHypurityTMAdvance,流动相为0.01mol/L磷酸-甲醇-四氢呋喃(86∶12∶2),检测波长为273nm。结果3组分检测浓度线性范围分别为0.39~100.0、1.96~500.0、1.17~300.0μg/ml,准确度均大于95.0%,日内、日间RSD均小于10.0%。结论该方法灵敏、准确、快速,特异性好,能满足散利痛3组分血药浓度测定要求。
OBJECTIVE:To establish a RP-HPLC method for simultaneous determination of caffine,acetaminophen and isopropylphenazone in human plasma after oral dose of saridon.METHODS:Hypersil Hypurity TM Advance was used as chromatographic column and the mobile phase was a mixture of0.01mol/L phosphoric acid-methanol-tetrahydrofuran(86∶12∶2).The detection wavelength was273nm.RESULTS:The calibration curves were linear in the ranges of0.39~100.0μg/ml for caffine,1.96~500.0μg/ml for acetaminophen,and1.17~300.0μg/ml for iso...
出处
《中国药房》
CAS
CSCD
北大核心
2006年第18期1395-1397,共3页
China Pharmacy