高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ的含量

Determination of Aristolochic Acid Ⅰ and Aristolochic Acid Ⅱ in Human Serum by HPLC

目的建立以高效液相色谱法测定人血清中马兜铃酸Ⅰ、Ⅱ含量的方法。方法色谱柱为C18(250mm×4.6mm,5μm),流动相为甲醇-水-冰醋酸(72∶27∶1),检测波长为250nm,流速为1.0ml/min。结果马兜铃酸Ⅰ、Ⅱ检测浓度线性范围分别为0.84~13.50μg/ml(r=0.9997,n=5)、2.03~32.50μg/ml(r=0.9994,n=5),最低检测浓度分别为0.3、0.1μg/ml。结论本方法简便、易行,可为临床诊治马兜铃酸肾病提供依据。

OBJECTIVE:To establish a HPLC method for the determination of aristolochic acidⅠand aristolochic acid Ⅱin human serum.METHODS:The separation was performed on C 18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-water-acetic(72∶27∶1)at a flow rate of1.0ml/min.The detection wavelength was250nm.RESULTS:The linear ranges for aristolochic acidⅠand aristolochic acid Ⅱ were0.84～13.50μg/ml(r=0.9997,n=5)and2.03～32.50μg/ml(r=0.9994,n=5),respectively.The lowest determination concentration of aristolochi...