摘要
目的:探讨益生注射液抗睾丸缺血再灌注损伤作用。方法:建立人尸睾丸离体缺血再灌注损伤模型,用益生注射液干预 后,观察睾丸形态,并以DNA原位缺口末端标记(TUNEL)技术显示生精细胞凋亡,NADPHd法显示一氧化氮合酶(NOS)活性,硝 酸盐还原酶法测定组织匀浆一氧化氮(NO)含量。结果:缺血再灌注使睾丸组织结构破坏,生精细胞凋亡指数增高,一氧化氮合酶 活性增强,一氧化氮合成增多(与正常对照相比,P分别小于0.01,0.05和0.01);益生注射液明显改善了睾丸组织形态,并使细胞凋 亡指数、一氧化氮合酶活性及一氧化氮生成量显著下降(与正常对照组相比,P均大于0.05)。结论:提示益生注射液可保持睾丸组 织中NO的正常含量,从而具有抗睾丸缺血再灌注损伤的作用。
OBJECTIVE To explore the protective mechanism and effect of Yisheng injection against isch emia-reperfusion injury (IRI) in human testes.METHODS Isolated human testes' IRI model was set up, and Yisheng injection was used in the experimental group. Histological changes were observed, TUNEL technology sho wed the apoptosis, activity of nitric oxide synthetase (NOS) was detected by NAD PHd method, and concentration of nitrous oxide (NO)was detected by nitrate red uctase method.RESULTS Ischemia-reperfusion...
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2005年第1期4-5,共2页
Chinese Journal of Hospital Pharmacy
基金
纽约中华医学基金会(CMB)基金资助(编号:2001 03 01)
关键词
缺血再灌注损伤
益生注射液
睾丸
一氧化氮
ischemia-reperfusion injury
Yisheng injection
testes
nitrous oxide
