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乳酸羟乙醇酸共聚物微球中蛋白包封率的测定 被引量：2

Determination of encapsulated protein in the PLGA microspheres
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摘要 目的:寻求一种合适的方法测定蛋白在乳酸羟乙醇酸共聚物(PLGA)微球中的包封率。方法:采用复乳 溶剂挥发法制备 BSA的PLGA微球,应用考马斯亮蓝法测定总蛋白浓度,根据文献报道的7种不同方法进行包封率测定。结果:不同的测定方法对 PLGA微球中真实的药物包封率的反映程度不同,相互间差异很大。结论:以水解法测定BSA在PLGA微球中包封率的方法提取 最完全。水解法中,又以乙腈作溶剂、再用氢氧化钠水解两步提取法最为简便、快速、准确。 OBJECTIVE To seek for an appropriate method for determining the amount of protein entrappe d in microspheres.METHODS PLGA microspheres containing bovine serum albumin (BSA) were made by double-emu lsion solvent evaporation. The total protein was analyzed by a coomassie brillia nt blue (CBB) staining method. Various determination methods of the BSA content in PLGA microspheres referred to in the literatures were performed in this work . RESULTS The content of protein in the same microspheres by differen...
作者 郑彩虹 梁文权 虞和永
机构地区 浙江大学药学院 浙江大学医学院附属妇产科医院
出处 《中国医院药学杂志》 CAS CSCD 北大核心 2005年第1期37-40,共4页 Chinese Journal of Hospital Pharmacy
基金 国家自然科学基金资助项目(编号:30171113)
关键词 牛血清白蛋白 乳酸羟乙醇酸共聚物 微球 BSA PLGA microspheres
分类号 R927 [医药卫生—药学]
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参考文献9

  • 1[1]Vila A,Sanchez A,Tobio M,etal. Design of biodegradable particles for protein delivery[J]. J Controlled Release, 2002, 78 (1～3):15.
  • 2[2]Yang YY, Chung TS, Ng NP. Morphology, drug distribution,and in vitro release profiles of biodegradable polymeric microspheres containing protein fabricated by double-emulsion solvent extraction/evaporation method[J]. Biomaterials, 2001,22 (3):231.
  • 3[3]Tuncay M, Calis S, Kas HS, et al. Diclofenac sodium incorporated PLGA (50 : 50) microspheres: formulation considerations and in vitro/in vivo evaluation [J]. Int J Pharm, 2000,195 (1～2):179.
  • 4[4]Castellanos IJ, Cruz G, Crespo R, et al. Encapsulation-induced aggregation and loss in activity of γ-chymotrypsin and their prevention[J]. J Controlled Release, 2002,81 (3):307.
  • 5[5]Gutierro I, Hemandez RM, Igartua M, et al. Influence of dose and immunization route on the serum IgG antibody response to BSA loaded PLGA microspheres [J]. Vaccine, 2002, 20 (17～18):2181.
  • 6[6]Cho KY, Choi SH, Kim CH, etal. Protein release microparticles ·based on the blend of poly(D, L-lactic-co-glycolic) and oligo-ethyl ene glycol grafted poly (L-lactide)[J]. J Controlled Release,2001, 76 (3):275.
  • 7[7]Diwan M, Park TG. Pegylation enhances protein stability during encapsulation in PLGA microspheres[J]. J Controlled Release,2001,73 (2):233.
  • 8[8]Li YP, Pei YY, Zhang XY, et al. PEG-lated PLGA nanoparticles as protein carriers: synthesis, preparation and biodistribution in rats[J]. J Controlled Release, 2001,71 (2):203.
  • 9[9]Rosas JE, Hernandez RM, Gascon AR, et al. Biodegradable PLGA microspheres as a delivery systerm for malaria synthetic peptide SPf 66[J]. Vaccine, 2001, 19 (31):4445.

同被引文献12

  • 1吕海鸿,廖海明,李勇,徐康森.RP-HPLC法测定酪丝亮肽中醋酸的含量[J].药物分析杂志,2004,24(3):286-289. 被引量：8
  • 2常国栋,李壮林,秦加阳,马翠卿,罗永章,许平.重组人血管内皮抑制素(rh-Endostatin)大肠杆菌表达体系发酵条件的优化[J].生物工程学报,2005,21(4):662-666. 被引量：26
  • 3朱艳,鲁莹,陈文娅,钟延强.胸腺肽α_1微球的制备及其评价[J].药学服务与研究,2005,5(3):248-250. 被引量：3
  • 4朱艳,鲁莹,钟延强.胸腺肽α_1缓释注射微球的研究[J].药学学报,2007,42(2):211-215. 被引量：6
  • 5Zhang H Y,Han Q W,Xu Y F,Fan Y R,Dai Q M,Xu J M,et al. The methods for determining the purity and in vitro or in vivo aetivity of recombinant human endostatin[J]. Cancer Biol Ther, 2005,4 : 207-212.
  • 6Kang J, Schwendeman S P. Comparison of the effects of Mg(OH)2 and sucrose on the stability of bovine serum albumin encapsulated in injectable poly (d, 1-1actide-co-glycolide) implants[J]. Biomaterials, 2002,23 : 239-245.
  • 7Barroug A,Kuhn L T,Gerstenfeld I. C,Glimcher M J. Interactions of cisplatin with calcium phosphate nanoparticles: in vitro controlled adsorption and release [J]. J Orthop Res, 2004,22: 703-708.
  • 8Zhang W,Walboomers X F,Jansen J A. The formation of tertiary dentin after pulp capping with a calcium phosphate cement, loaded with PLGA microparticles containing TGF-betal [J]. J Biomed Mater Res A,2008,85:439-444.
  • 9Fei Z,Hu Y,Wu D,Wu H,Lu R,Bai J, et al. Preparation and property of a novel bone graft composite consisting of rhBMP-2 loaded PLGA microspheres and calcium phosphate eement[J]. J Mater Sci Mater Med,2008,19:1109-1116.
  • 10Zhi Yao, Rong Lu, Jing Jia, et al. The effect of tripeptide tyroserleutide (YSL) on animal models of hepatocarcinoma [J]. Peptides, 2006,27(6) : 1167 1172.

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二级引证文献8

中国医院药学杂志
2005年 第1期

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