毛细管气相色谱法测定癫痫患儿血清中丙戊酸浓度

Capillary column gas chromatography determination of serum valproic acid in epil eptic children

目的:建立血清中丙戊酸(VPA)毛细管气相色谱测定方法,监测丙戊酸血药浓度,分析血药浓度与剂量及疗效关系。方 法:程序升温高效毛细管气相色谱法测定癫痫患儿血清中丙戊酸浓度。结果:丙戊酸在20～300mg·L-1范围内线性关系良好,最 低检测浓度为1mg·L-1。相对回收率96%以上,日内RSD<4.3%,日间RSD<7.6%。23例癫痫患儿血药浓度个体差异大,其中 13例患儿剂量(20.8±3.8)mg·kg-1·d-1,血药浓度为(53.8±19.1)mg·L-1;10例患儿剂量(32.6±2.6)mg·kg-1·d-1,血药浓度 为(65.3±27.8)mg·L-1。结论:本法专属性强、简便、快速、准确,适合于体液中丙戊酸的监测及药动学研究;癫痫患儿口服丙戊酸 钠血药浓度个体差异大,血药浓度与给药剂量无显著相关性,宜采用个体化用药。

OBJECTIVE To establish a method for the determination of valproic acid (VPA) in serum ,monitore serum concentration of VPA in epileptic children,and investigate the relations of VPA serum concentration to dosage and effect of treatment in epil eptic children.METHODS The concentration of VPA were measured by capillary column gas chromatography a ssay, the column temperature was risen by program.RESULTS The linear relation of VPA were in the range of (20-300) mg ·L -1.Th e delection limit of VPA in...