摘要
目的:从细胞学水平探讨抗纤软肝冲剂抗肝纤维化的作用机制.方法:采用酶消化法、密度梯度离心法,分离正常大鼠肝星状细胞(HSC),培养、鉴定及传代.制备抗纤软肝冲剂药物血清,温育传一代HSC,并设生理盐水组和秋水仙碱组为对照.3H-proline掺入法测定细胞活力;3H-TdR掺入法和MTT比色法测定细胞增殖.结果:抗纤软肝冲剂组药物血清对细胞形态无影响,还能明显提高细胞的3H-proline掺入(P＜0.01),明显抑制细胞的3H-TdR掺入量和MTT转化(P＜0.01),且对MTT的抑制作用呈浓度依赖性递增趋势.结论:抗纤软肝冲剂能提高细胞活力,无细胞毒性作用;能抑制HSC的增殖,这可能是该方抗肝纤维化的细胞学机制之.
Objective:To investigate the antifibrotic mechanism of kangxian Ruangan Granula(KXRGG) at cellular lever. Method:HSC were isolated from the normal rats by enzyme digestion and density gradient centrifugation, cultured, identified and subcultured. The medicated sera were prepared to incubated with the subcultured HSC. Sodium chloride sera and colchicines sera were taken as control groups. HSC viability was observed by H-proline; cell proliferation was measured with H-TdR incorporation and MTT colorimetric as...
出处
《中药材》
CAS
CSCD
北大核心
2001年第11期809-811,共3页
Journal of Chinese Medicinal Materials
基金
湖北省教委基金(96A027)
关键词
抗纤软肝冲剂/血清药理学
肝星状细胞
增殖
肝纤维化
Kangxian Ruangan Granula
Serum pharmacology
Hepatic stellate cell
Proliferation
Hepatic fibrosis
