摘要
CZE ESI MS is one of the most sensitive detection method and one of the potential method for protomics analysis. In this work, peptide mixture was separated and detected by using CZE ESI MS, and the influence of pH value of buffer and composition of sheath liquid on CE separation and MS detection was investigated. The method allows the detection limits up to 4 2—33 pg. The result of this method is better than that of CE UV for the peptide analysis.
CZE ESI MS is one of the most sensitive detection method and one of the potential method for protomics analysis. In this work, peptide mixture was separated and detected by using CZE ESI MS, and the influence of pH value of buffer and composition of sheath liquid on CE separation and MS detection was investigated. The method allows the detection limits up to 4 2—33 pg. The result of this method is better than that of CE UV for the peptide analysis.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2004年第3期448-450,395,共4页
Chemical Journal of Chinese Universities
基金
国家重点基础研究发展规划项目 (批准号 :0 0 1CB5 10 2 0 2 )资助
关键词
毛细管电泳
肽
质谱
包层液
电喷雾接口
Capillary electrophoresis
Peptide
Mass spectrometry
Sheath liquid
Electrospray ionization interface
