组织型纤溶酶原激活剂重组DNA的细菌克隆

Bacterial Cloning of Recombinant DNA in Expression of t-PA

采用Ca（2+）处理大肠杆菌RR1进行转化实验，使含组织型纤溶酶原激活剂（t－PA）cDNA的重组质粒转化入细菌进行克隆获得成功，经氨苄青霉素（AP）培养基筛选及限制性内切酶鉴定，证明转化子为阳性。

We have successfuly transformed DNA of tissue type plasminogen activator in Escherichia coli (Ecoli)RRI treated by screening test on LB-agar plate with ampicillion and upon analysis with restriction endonucleases. The results has a practical significance for further research on expression of t-PA both in E coli and eukaryticcells.