发酵液中色氨酸含量的快速测定

Quick Determination of L-Tryptophan in Fermented Broth

报道了一种利用分光光度法快速测定发酵液中色氨酸的新方法。该方法基于在硫酸存在下 ,色氨酸与对二甲胺基苯甲醛缩合生成蓝色化合物 ,该产物在 60 0nm处有吸收峰 ,对色氨酸测定的范围为 0～ 1 0 0 μg/mL ,工作曲线方程为 C =2 3 .5 6A ,C为色氨酸 ,A为吸光度。相关系数为R2 =0 .9972。

A new spectrophotometric method to determine L-tryptophan in fermented broth is reported. Tryptophan can be condensed with p-dimethylamino-benzaldehyde in the presence of sulfuric acid and a blue complex is formed. The maximum absorption of the complex is at 600nm. The linear scale is 0～100μg/mL,and the equation of the standard curve is C(Tryptophan)=23.56A, where A represents absorbance. Coefficient of correlation R 2 is 0.9972。