RT-PCR检测氧化砷治疗APL复发患者的PML-RARα融合基因

PML-RAR α detection by RT-PCR in relapsed APL patients treated with arsenic trioxide (AS_2O_3)

目的：探讨急性早幼粒细胞性白血病（ＡＰＬ）患者在氧化砷（Ａｓ２Ｏ３）治疗过程中，ＰＭＬ－ＲＡＲα融合基因的变化。方法：应用ＲＴ－ＰＣＲ技术动态观察１４例ＡＰＬ复发患者在Ａｓ２Ｏ３治疗过程中，骨髓单个核细胞中ＰＭＬ－ＲＡＲα转录本的改变。结果：检测的１１例诱导缓解患者中，９例存在阳性ＰＭＬ－ＲＡＲα转录本，随访的６例患者中，３例呈现ＰＭＬ－ＲＡＲα转阴，但２例分别在缓解后５、７个月转阳。

Objective:To appraise the alteration of PMLRARα fusion gene as a prognostic marker in acute promyelocytic leukemia (APL) treated with arsenic trioxide (As_2O_3). Methods: RT-PCR was used todetect PML-RARα transcripts in mono nuclear cell of bone marrow from 14 cases with relapse. Results: On remission, 9 out of 11 patients tested still showed positive PML-RARα transcripts; during following-up,3 out of 6 patients turned negative, one each remained positive at 5 and 7 months after remission, respectively. Co...