摘要
目的探讨可用于诊断小肠移植后排斥反应的指标。方法采用F334大鼠建立同系全小肠移植模型,以近交系F334大鼠和BN大鼠建立同种全小肠移植模型,术后采用流式细胞仪连续测定外周血CD4+、CD8+细胞中CD45+亚群以及CD45RC+细胞与CD45RC-细胞比值(CD45RC+/CD45RC-)的变化,同期进行移植肠组织病理学检查。结果同系移植组受者术后均获长期存活(>28d);同种移植组受者术后存活(12.0±1.3)d,出现中、重度排斥反应。术后外周血CD4+CD45+和CD8+CD45+细胞的变化,两组间的差异无统计学意义;同系移植组CD4+CD45RC+/CD4+CD45RC?及CD8+CD45RC+/CD8+CD45RC?在术后3d升高,然后迅速降至手术当天的水平,同种移植组上述指标术后均维持较高水平。结论动态观察CD4+CD45RC+/CD4+CD45RC—较适于监视排斥反应的发展过程,而CD8+CD45RC+/CD8+CD45RC—更适于排斥反应的早期诊断。
Objective To investigate the probable markers for rejection diagnosis following small bowel transplantation. Methods The models of isogenic and allogenic whole small bowel transplantation were established using inbred rats, while rats F344 served as donors and recipients in isotransplantation and rats F344 and rats BN served as donors and recipients respectively in alltransplan-tation. Postoperatively, the changes of CD45+ subsets and the ratios of CD45RC+/CD45RC-in CD4+ and CD8+ lymphocytes in recipients’ ...
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第7期404-407,共4页
Chinese Journal of Organ Transplantation
基金
天津市自然科学基金资助(983601811)
关键词
移植
小肠
移植物排斥
抗原
CD45
大鼠
Transplantation
Intestine
small
Graft rejection
Antigens
CD45
Rats
