手机微波辐照对人晶状体上皮细胞DNA的损伤作用及其对细胞增殖活性的影响

DNA damage and repair induced by acute exposure of microwave from mobile phone on cultured human lens epithelial cells

目的探讨手机微波对人晶状体上皮细胞(humanlensepithelialcells,hLECs)DNA的损伤作用及其对细胞增殖活性的影响。方法将hLECs分为辐照A、B、C、D组和假照组,置于sXc-1800细胞辐照系统内连续辐照2h,辐照强度[比吸收率(specificabsorptionrate,SAR)]辐照A、B、C、D组分别为1.0、2.0、3.0、4.0W/kg,辐照后0.0、0.5、1.0h分别进行彗星实验,检测细胞DNA的损伤及其修复情况。微波辐照后在培养液中加入溴脱氧尿苷(BrdU),继续培养1h后采用BrdU原位检测试剂盒显色,观察细胞的增殖率。结果辐照A、B组细胞DNA损伤与假照组比较,各个检测时段的差异均无统计学意义(P>0.05);辐照后即刻辐照C、D组DNA损伤与假照组比较,差异有统计学意义(P<0.01),修复0.5h后差异仍有统计学意义(P<0.05),修复1h后辐照C组的差异消失(P>0.05),而辐照D组的差异持续存在(P<0.01)。BrdU原位检测试剂盒显色结果表明辐照A、B、C组细胞增殖情况无明显变化(P>0.05),辐照D组细胞增殖率降低,差异有统计学意义(P<0.01)。结论SAR≤3W/kg的1.8GHz手机微波短期急性作用对体外培养的hLECs的DNA不产生或产生可修复的损伤,对其后的细胞增殖情况亦无影响,4.0W/kg的辐照剂量可导致细胞DNA不可逆性损伤,使细胞的增殖率下降。

Objective To investigate the effects of acute exposure of low-power 217 Hz modulated 1.8 GHz microwave radiation on the DNA damage of human lens epithelial cells (hLECs) and repair. Methods Cultured hLECs were exposed to 217 Hz modulated 1.8 GHz microwave radiation at SAR (specific absorption rate) of 1.0, 2.0, 3.0 and 4.0 W/kg for 2 hours in an sXc-1800 incubator and irradiate system, the DNA single strand breaks were detected with comet assay (single-cell gel electrophoresis) in sham-irradiated cells and ...