粘虫颗粒体病毒增效因子的基因定位

Location of the gene encoding synergistic factor of the Pseudaletia unipuncta granulosis virus

参考粉纹夜蛾Trichoplusiani颗粒体病毒增强因子的基因序列 ,设计PCR引物 ,用PCR反应扩增出特异性产物。用EcoRⅠ、BamHⅠ双酶酶切处理PCR反应产物 ,然后克隆到质粒pUC19中 ,构建重组质粒 pUC19 SF ;对重组质粒 pUC19 SF中的外源片段测序 ,结果证明PCR扩增产物是粘虫颗粒体病毒PuGV Ps增效因子基因的一段序列。重组质粒pUC19 SF的插入片段标记为探针 ,通过Southern杂交将增效因子基因定位于PuGV

Using the nucleotide sequence of the VEF (viral enhancing factor) gene of Trichoplusia ni granulosis virus for reference, we designed a primer for PCR and amplified a specific fragment by PCR reaction. A recombinant plasmid pUC19 SF was constructed by cloning the PCR product which was treated with Eco RⅠ, Bam HⅠ into pUC19. The PCR product was proven to be a fragment of the gene encoding the synergistic factor of Pseudaletia unipuncta granulosis virus (PuGV Ps) by DNA sequencing. After labeli...