水稻抗白叶枯病近等基因系CBB30的培育及Xa30(t)的初步定位

Breeding of Near-Isogenic Line CBB30 and Molecular Mapping of Xa30(t), a New Resistance Gene to Bacterial Blight in Rice

【目的】将普通野生稻资源Y238所携有的抗白叶枯病新基因Xa30(t)导入栽培稻JG30,培育近等基因系,进行分子标记定位,以便应用于育种实践。【方法】以感病籼稻品种JG30为轮回亲本,Y238为供体进行杂交、回交、自交培育近等基因系;以JG30/Y238的一个BC6F2代群体为定位群体,利用分离集团分析法(BSA),借助SSR、EST、STS等分子标记对Xa30(t)进行分子标记定位。【结果】成功培育了携有Xa30(t)基因的水稻抗白叶枯病近等基因系CBB30。从343个分子标记中筛选出4个能揭示抗感多态性的标记RM1341、V88、C189及03STS,用该4个标记对BC6F2代群体303个单株进行分子检测和连锁分析,结果表明上述4个标记均位于水稻第11染色体长臂,与Xa30(t)的遗传距离分别为11.4cM、11.4cM、4.4cM及2.0cM,且它们位于Xa30(t)基因的同一侧。【结论】通过构建近等基因系及分子标记检测找到4个与Xa30(t)基因连锁的标记RM1341、V88、C189及03STS,将Xa30(t)基因定位于水稻第11染色体长臂上。

【Objective】A new rice bacterial blight resistance germplasm (Y238) was identified from the wild rice species Oryza rufipogon and the resistance locus was transferred to the cultivated rice to breed near-isogenic line. Molecular mapping of the resistance gene was carried out to put it into practical use in the future. 【Method】The resistance gene Xa30(t) in Y238 has been introduced into JG30 by cross, backcrosses and selfing. A BC6F2 population was used for molecular mapping of Xa30(t), and Bulked Segregant A...