摘要
Phenothalein deoxyribonucleic acid(DNA) cetyltrimethyl ammonium bromide(CTMAB) system has been studied by resonance light scattering(RLS). In Tris HCl buffer solution(pH=9 30), phenothalein and DNA react with CTMAB to form large particles of ion association complex, which results in significant enhancement of RLS intensity. The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated. Under optimum conditions, the enhanced RLS intensity was proportional to the concentration of DNA in the range of 0 04 to 0 8 mg/L for fish sperm DNA( fsDNA ) and 0 03 to 2 5 mg/L for calf thymus DNA(ctDNA). The detection limits were 12 0 μg/L and 17 0 μg/L for fsDNA and ctDNA, respectively.
Phenothalein deoxyribonucleic acid(DNA) cetyltrimethyl ammonium bromide(CTMAB) system has been studied by resonance light scattering(RLS). In Tris HCl buffer solution(pH=9 30), phenothalein and DNA react with CTMAB to form large particles of ion association complex, which results in significant enhancement of RLS intensity. The spectral characteristics of RLS, the effective factors and optimum conditions of the reaction have been investigated. Under optimum conditions, the enhanced RLS intensity was proport...
出处
《应用化学》
CAS
CSCD
北大核心
2004年第11期1187-1189,共3页
Chinese Journal of Applied Chemistry
基金
湖南省自然科学基金(00JJY2084)
湖南省教育厅基金(02C462)资助项目
