太湖梅梁湾冬季浮游细菌的多样性

Bacterioplankton diversity in Meiliang Bay of Taihu Lake in winter.

运用分子生物学方法对太湖梅梁湾2005年3月冬季水体中的浮游细菌多样性进行了研究。提取水样中细菌基因组总DNA,以细菌的16SrDNA通用引物进行PCR扩增,扩增产物经分子克隆、酶切归类分析、测序和序列分析,对水样中的细菌群落建立了针对16SrDNA的克隆文库和系统发生树。基于细菌16SrDNA克隆文库和系统发生树的分析,结果表明,该水域的优势细菌类群为拟杆菌(Cy-tophaga-Flavobacterium-Bacteroides,44.3%)和β、γ变形杆菌(Proteobacteria;β-Proteobacteria,25.3%和γ-Proteobacteria,27.8%),其中大部分细菌与黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)和食酸菌属(Acidovorax)细菌的关系密切。与国外富营养化湖泊的研究报道相比较,γ-Proteobacteria类群为水体中的优势类群,是严重富营养化的太湖在冬季所具有的独特种群结构特征。

In this paper,the bacterioplankton diversity in the water body of Meiliang Bay in March,2004 was studied by using the techniques of molecular biology,including the extraction of bacterial DNA,PCR amplification of bacterial 16S rDNA by universal primers,molecular clone,RFLP analysis,sequencing of 16S rDNA fragments,and sequence analysis. The clone library aimed at 16S rDNA and the phylogenetic tree were constructed. The analysis of sequencing results based on 16S rDNA indicated that the dominant bacterial gr...