摘要
我们的前期研究发现运动性肥大心脏心肌血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）受体数目减少，但对这种变化的调控是否可发生在基因转录水平上目前尚不清楚。本实验采用反转录-聚合酶链式反应（ReverseTranscription－PolymeraseChainReaction，RT－NR）方法对运动性肥大心脏心肌AngⅡAT1受体工型mRNA表达进行研究。结果表明大鼠游泳运动8周后，心系数比对照组大鼠提高24％（p＜0．05）。琼脂糖电泳结果表明心肌AbgⅡAT1受体亚型RT－PCR产物条带密度比对照组降低30％，面积降低61％（p＜0．05）。说明运动性肥大心脏心肌AngⅡAT1受体基因表达降低。提示运动性肥大心脏心肌AngⅡ受体数目减少的调控机制可发生在基因转录水平上。
Our previous studies showed that the number of angiotensinⅡ (Aug Ⅱ ) receptors was decreased in exercise - induced hypertrophied rat heart. However it is still not clear that number decrease of Ang Ⅱ receptor is due to downregulation in its gene expression. We therefore studied the expression of AngⅡ AT1 receptor subtype mRNA by Reverse Transcription - Polymerase Chain Reaction (RT - PCR). The Wistar rats subjected to an 8 - weeks swiming program and increased their heart weight/boby weight by24% than that ...
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
1999年第3期208-210,共3页
Chinese Journal of Sports Medicine
基金
北京市自然科学基金!7952015
关键词
血管紧张素Ⅱ受体
基因表达
心肌肥大
运动
angiotensin Ⅱ
AT_1 receptor subtype
gene expression
cardiac hypertrophy
exercise
