两种白斑小鼠突变基因的染色体定位

Two Kinds of White Spot Mice and Mapping of Their Mutation Genes

以本中心ENU诱变获得的两种白斑突变小鼠W-4Bao与Kitl-1Bao为研究对象[均为C57BL/6J(B6)背景],遗传试验表明它们都为单基因显性遗传,W-4Bao及Kitl-1Bao突变基因纯合子小鼠的表型分别为全白色及“黑头白”;将白斑杂合子小鼠与DBA/2(D2)交配获得具有白斑表型的F1小鼠,F1小鼠再回交D2繁殖[(B6×D2)F1×D2]F2小鼠,利用微卫星标记对F2代小鼠进行连锁分析。结果发现W-4Bao与微卫星D5Mit356、D5Mit308之间的LOD值分别为56.82、51.50,从而把该突变基因定位于第5号染色体D5Mit356与D5Mit308之间;Kitl-1Bao与微卫星D10Mit70、D10Mit68之间的LOD值分别为27.37、21.20,从而把该突变基因定位于第10号染色体上D10Mit70与D10Mit68之间。经过检索小鼠基因组数据库确认它们的候选基因分别为kit及kitl。

Two kinds of white spot mutant mice,W^(-4Bao) and Kitl^(-1Bao),obtained by ENU mutagenesis were the material in this study.Heredity tests indicated that the phenotypes of the two white spot mice were controlled by different single dominant gene and the phenotypes of homozygote mice were white and black head white respectively.To map the mutation genes of the two kinds of white spot mutant mice,microsatellite markers and linkage analysis were used to detect F_2 mice,which were obtained by back-crossing(DBA/2...