摘要
福尔马林被广泛应用于生物标本的长期保存。由于福尔马林可能影响标本DNA的质量,因此需要对福尔马林固定标本DNA的提取和扩增过程进行改进。影响从福尔马林保存标本中提取的DNA质量的主要因素包括福尔马林导致的DNA与蛋白质之间、蛋白质与蛋白质之间、DNA与DNA之间的交联,福尔马林溶液的化学成分、pH值及浓度,标本保存的时间和温度,标本保存部位等。本文总结了目前常用的对标本DNA提取和扩增过程的改进措施及其优点。
Formalin-fixation is commonly employed in the practices of the convenient and long-term storage of museum specimens.Thus,there is a need for method by which high quality and quantity DNA could be extracted from formalin-fixed tissue.Factors that affect DNA extraction and amplification from formalin-fixed samples are discussed in this paper.Some of the most important factors are considered to be: the formaldehyde-mediated DNA-protein,protein-protein and DNA-DNA cross-linkings,the DNA adducts with histone,the...
出处
《四川动物》
CSCD
北大核心
2006年第3期662-665,共4页
Sichuan Journal of Zoology
关键词
福尔马林
标本DNA
提取
扩增
formalin
specimen DNA
extraction
amplification
