摘要
分别获得CSF1PO,TPOX和TH013个短串联重复序列STR,对新疆锡伯族和哈萨克族人群中等位基因频率、基因型频率及相关法医学数据进行比较。应用PCR技术、4%变性聚丙烯酰胺凝胶电泳及银染技术对上述3个STR位点分型。锡伯族人群CSF1PO位点有9个等位片段,TPOX位点有8个等位片段,TH01位点有8个等位片段;哈萨克族人群CSF1PO位点有8个等位片段,TPOX位点有8个等位片段,TH01位点有7个等位片段,两民族各自的3个STR位点的基因型分布均符合Hardy-Weinberg平衡;锡伯族人群各位点杂合度分别为0.9426,0.8361,0.8853,多态信息量分别为0.8298,0.7213,0.7626;哈萨克族人群各位点杂合度分别为0.8753,0.8777,0.9321,多态信息量分别为0.7401,0.7568,0.7509。以上3个STR位点的基因频率分布在两个不同的人群中具有显著的差异。
The purpose is to obtain allele frequency, genotype frequency and related forensic data of CSF1PO,TPOX and TH01 loci in Chinese Xingjiang Xiao population and Chinese Xingjiang Hasake population. Genomic DNA from peripheral blood mononuclear cells of normal Chinese Xinjiang Xibo population and Chinese Xinjiang Hasake population was used as template and CSF1POP TPOX and TH01 fragments were amplified by polymerase chain reaction (PCR). The PCR products were analyzed by 4% denaturing PAGE and detected using si...
出处
《西北大学学报（自然科学版）》
CAS
CSCD
北大核心
2002年第5期564-568,共5页
Journal of Northwest University（Natural Science Edition）
基金
国家自然科学基金资助项目(39970401)
关键词
短串联重复序列
遗传多态性
聚合酶链反应
电泳
short tandem repeats
genetic polymorphism
polymerase chain reaction
electrophoresis
