粗淀粉原料谷氨酸发酵新工艺研究

A NEW TECHNOLOGY OF GLUTAMIC ACID FERMENTATION ON CRUDE STARCH

本研究采用木薯粗淀粉原料，双酶法制糖，在过量生物素下添加青霉素的发酵工艺路线，以接种量８％、初糖浓度１００～１３０ｇ／Ｌ，在发酵的适当时间添加适量青霉素（约４ｕ／ｍｌ），发酵过程流加尿素溶液控制ｐＨ７．１～８．０范围，同时流加浓糖液，经４０小时摇瓶培养，结界谷氨酸生成量高达１０１．９ｇ／Ｌ，糖酸转化率５９．５３％。与传统的采用精淀粉控制生物素＂亚适量＂工艺相比，可大幅降低粮耗、降低成本，提高产酸１５～２０ｇ／Ｌ．

This new GA fermentation technology features adding penicillin into biotin-excessive broth.The cassava crude starch was adopted as a raw material which was hydrolysed by two enZymes. In the process of fermentation,the pH was controled by feeding urea. High concentration glucose was fed to supplement suger.The optimum conditions were obtained when innoculation amount was 8%, penicillin added about 4u/ml at suitable time, pH7.1￣ 8.0, after 40 hour incubation in shaking bottle,GA yielding rate was up to 101.7g...