SOD与酪氨酸酶共存时各自的活性

The Respective Activeties of Coexisted SOD and Tyrosinase

本文采用NBT(氮兰四唑)光还原法,测定了在酪氨酸酶存在下SOD酶的活性,并采用生成DOPA红的方法测定了SOD酶存在下酪氨酸酶的活性.并做了一系列干扰实验,实验结果指出:在酪氨酸酶存在下SOD催化O_2~??+O_2~??+2H^+??H_2O_2+O_2的反应活性降低,而在SOD存在下酪氨酸酶催化DOPA生成DOPA红的反应有明显抑制作用.

Superoxide dismutase (SOD) and tyrosinase are two important copper enzymes with their catatytic centers being copper, ligands that can coordinate with cop-perions may inhibit the catatytic activities of copper enzymes to varying extent.In order to get a clear understanding of the effects on the activities of both enzymes when they are coexisted, the activities of SOD are determined in the presence of tyrosinase with the method of photoreducing NBT(nitro blue tetrazolium)and the activities of tyrosinase are ...