黄精不同炮制品中5-羟甲基糠醛的含量测定

Determination of 5-Hydroxymethyl Furfuraldehyde in Different Processed Products of Polygonatum

目的:通过对不同炮制时间5-羟甲基糠醛含量变化规律的考察,为黄精的炮制工艺及质量标准研究奠定基础。方法:超声提取法获得供试品溶液,HPLC法测定其含量;色谱条件:分析柱Agilent HC-C18柱(5μm,250mm×4.6 mm),甲醇-水(15∶85)为流动相,流速为1 ml/min,检测波长为284 nm。结果:黄精不同炮制品中都检测出5-羟甲基糠醛,蒸制30 h内含量基本稳定,但受热30 h以后含量急剧上升,继续加热含量下降。结论:该结果能够为黄精质量控制和饮片加工与规范化炮制提供参考数据。

Objective: By finding the changing regularity of 5-Hydroxymethyl furfuraldehyde(5-HMF) content in Polygonatum for different time,this provides basis for the technology of processing Polygonatum and quality standards of its prepared pieces.Methods: The testing solution was acquired by ultrasonic extraction,HPLC was used to determine the contents.The chromatographic conditions: Agilent HC-C18 column(5 μm,250 mm×4.6 mm).The mobile phase: methanol-water(15∶85) at the flow rate of 1 ml/min.The determination wave...