戴氏虫草多糖抗突变活性的研究

Study on the Antimutagenicity of Cordyceps taii Polysaccharide

目的:探讨戴氏虫草菌丝体胞外水溶性多糖(CDP1)是否具有抗突变效应。方法:采用Am es试验及小鼠骨髓嗜多染红细胞(PCE)微核形成试验评价不同浓度的CDP1的抗突变作用。结果:CDP1在未加S9条件下,对TA97、TA98、TA100及TA102回复突变均具有不同程度的抑制作用,尤其对TA97、TA98及TA102更为明显,其最高抑制率分别达68.5%、79.5%及81.3%,且结果呈现一定的剂量-效应关系,对TA100表现较弱,但最高抑制率仍达19.8%;CDP1对环磷酰胺诱发的小鼠骨髓PCE微核发生率也有显著的拮抗作用,其最高抑制率达70.3%,且结果亦呈现剂量-效应关系。结论:在本实验条件下,戴氏虫草菌丝体胞外水溶性多糖具有明显的抗突变活性。

Objective: To study the antimutagenicity of Cordyceps taii polysaccharide(CDP1).Methods: Ames test and micronucleus form assay of polychromatic erythrocytes in mice bone marrow were used to evaluate the antimutagenic effect of CDP1 with different concentrations.Results: CDP1 could inhibit the reverse mutation on Salmonella typhimuriun strains TA97,TA98,TA100 and TA102 without S9 in different degree,especially remarkable in the strains T97,T98 and T102.The maxium inhibition rates of strains TA97,TA98,TA100 a...