四物汤传统饮片汤剂与配方颗粒汤剂中阿魏酸、芍药苷含量比较

Comparison of Ferulic Acid and Paeoniflorin between Traditional Slice Decoction and Dispensing Granule Decoction of Siwu Tang

目的:比较四物汤传统饮片汤剂与配方颗粒汤剂中阿魏酸、芍药苷的含量。方法:采用高效液相色谱法。色谱柱:Diamonsil C18(4.6 mm×250 mm,5μm);柱温:35℃;流速1 ml/min;测定阿魏酸流动相为甲醇-水-0.05%磷酸(40∶50∶10),检测波长为327 nm;测定芍药苷流动相为乙腈-水-0.05%磷酸(16∶74∶10),检测波长为230 nm。结果:自制配方颗粒汤剂中阿魏酸含量为8.91 mg/剂,芍药苷为83.57 mg/剂;传统饮片汤剂中阿魏酸含量为8.90 mg/剂,芍药苷为78.51 mg/剂;不同厂家配方颗粒汤剂中阿魏酸含量为3.36~7.67 mg/剂,芍药苷为48.13~67.52 mg/剂。结论:四物汤配方颗粒汤剂与传统饮片汤剂的色谱图基本一致,自制配方颗粒汤剂与传统饮片汤剂中阿魏酸和芍药苷含量基本相当,但不同厂家配方颗粒汤剂中存在显著性差异。

Objective: To compare the contents of ferulic acid and paeoniflorin between traditional slice decoction and dispensing granule decoction of Siwu Tang. Methods: A HPLC method detected ferulic acid and paeoniflorin with Diamonsil C18(4.6 mm×250 mm,5 μm) column.The column temperature was setup at 35℃ and the flow rate was 1 ml/min.The mobile phase for ferulic acid was a mixed solution of MeOH-H2O-0.05% H3PO4(40∶50∶10) and the wavelength was 327nm,while for paeoniflorin was a mixed solution of CH3CN-H2O-0.05% H...