金铁锁不同居群rDNA ITS序列分析

Ribosomal DNA ITS Sequence of Analysis of Psammosilene tunicoides from Different Populations

目的:分析金铁锁不同居群的核糖体ITS碱基序列,为鉴别不同产地金铁锁提供分子依据。方法:使用1对引物18P1和26P2进行ITS基因的PCR扩增并测序。结果:12个居群金铁锁的ITS1片段长度为225~229 bp,ITS2片段长度为166~170 bp,5.8S片段长度为261~264 bp。云南昆明、丽江、个旧、鹤庆和四川盐源5个居群的ITS序列碱基完全一致,云南宣威、会泽、中甸、保山、四川木里、西藏林芝等7个居群的ITS序列则有不同的变化,碱基变异数目(包括5.8 S编码区)为1~4个。结论:经过比较分析,rDNA ITS区碱基序列在分布于云南中部、四川西南端等居群与云南西部、西北端、西藏东南部、四川西南部居群具有各自相应的指纹特征,可以作为相应居群的鉴别依据。

Objective: To analyze the ribosomal ITS sequence variation of Psammosilene tuncolides W.C.Wu et C.Y.Wu from different populations,for identifying different local populations.Methods: A pair of primers of 18SP1 and 26SP2 with PCR technique had been applied to study the ITS sequences.Results: The sequences of ITS1,ITS2 and 5.8S are 225~229 bp,166~170 bp and 261~264 bp.Among 12 local populations,the sequence of Kunming,Lijiang,Gejiu,Heqing of Yunnan and Yanyuan of Sichuan showed no variation,there were 1~4 var...