摘要
本文对光学技术在细胞成像方面的应用进行了综述。着重介绍了激光扫描共聚焦显微镜、多光子荧光成像技术、全内反射荧光显微镜、近场扫描光学显微成像技术、光学相干层析成像技术的基本原理及其主要特点。体现了光学检测技术无侵入、无电离辐射,具有多种操作模式,可获得实时与定量等多种信息的特点,在生命科学研究中具有举足轻重的地位。
Optical technology is very important for the cell imaging. Several microscopies, i.e. Laser Scanning Confocal Microscopy(LSCM), Multiphoton Fluorescence Microscopy(MFM),Total Internal Reflection Fluorescence Microscopy(TIRFM), Near-field Scanning Optical Microscopy(NSOM), Optical Coherence Tomography(OCT), are reviewed in this paper. The principle and the main characters of these microscopies are briefly introduced. And it is showed that optical technology is noninvasive and non-radiate, with multi-operatio...
