罗汉果中三萜皂苷的含量测定

The colorimetric determination of Triterpenoid saponin in Luohanguo

研究了罗汉果中三萜皂苷的比色测定方法,试验证明:用香草醛-高氯酸为显色剂的最佳条件为:加入待测试样后,加热挥去溶剂,再加入0.2mL新制的5%香草醛-冰醋酸溶液及0.8mL高氯酸试剂, 在60℃恒温水浴加热15min,冷却,摇匀,在波长为595nm处测定。该方法操作简单,灵敏度高且比较准确。

Colorimetric determination of Triterpenoid saponin in Luohanguo was investigated in the present work. Experimental results revealed that the optimal condition was determined when vanillin-perchloric acid served as a corloring reagent. Added samples were warmed up for drying up. 0.2mLof freshly prepared 5%vanillin in acetic solution and 0.8mL of perchloric acid were then add. The mixture was warmed up in a water bath at 60℃ for 15min. After cooling down and well shaken, and samples were measured colorimetric...