藏药短管兔耳草中松果菊苷和麦角甾苷的RP-HPLC分析

A RP-HPLC Method for Simultaneous Determination of Echinacoside and Acteoside in Tibetan Herb Lagotis brevituba Maxim

建立了藏药短管兔耳草中松果菊苷和麦角甾苷含量的高效液相色谱分析法。采用Waters XTerra RP18色谱柱(150mm×4.6mm,5μm),以甲醇-1%冰醋酸溶液(28:72,V:V)为流动相,流速1.0mL/min,检测波长330nm,柱温30℃,在20min内分离检测了该两种化合物。松果菊苷和麦角甾苷进样量分别在0.077~4.950μg(r=0.9999)和0.085~5.450μg(r=0.9999)内呈良好线性,平均加样回收率分别为98.35%和92.50%,RSD分别为2.35%和2.86%。所建立的方法简便、快捷、结果准确可靠,重现性好,可用于藏药短管兔耳草的质量控制,并为兔耳草属植物中苯丙素苷类化合物的分离分析提供一定的参考。

To establish RP-HPLC method coupled with photodiode array detection (PAD) for the determination of echinacoside and acteoside in Tibetan herb Lagotis brevituba Maxim,well separation was achieved with in 20 min on a Waters XTerra RP18 column (150 mm×4.6 mm,5 μm) using methanol-1% acetic acid solution(28:72,V:V)as the mobile phase at a flow rate of 1.0 mL/min and column temperature 30 ℃,330 nm as the detection wavelength.Echinacoside showed good relationship at the range of 0.077-4.950 μg(r=0.999 9),and acteo...