人群脑膜炎奈瑟菌带菌检验方法的研究

Studies on the methods for detecting carriers of N.meningitidis in the crowd

目的:研制一种能够在低温下保持脑膜炎奈瑟菌活性并具有增菌作用的培养基。方法:配制一种小牛血清"双抗"培养基,用已知量A、C群脑膜炎奈瑟菌检定其保菌和增菌效果,并用该法在正常人群中检测带菌率,以常规方法的检测结果作配对比较。结果:应用小牛血清"双抗"培养基可使脑膜炎奈瑟菌在4℃24小时内基本不死亡,部分菌株可生存8天,同时可使存活的细菌从3～9 CFU/ml增加到(6～7)×107 CFU/ml;对364名正常人检测,其检出率达1.65%,而常规方法检出率为0(P<0.05)。结论:小牛血清"双抗"培养基在4℃的不利条件下,有显著保菌作用,同时具有显著增菌作用。

Objective: To manufacture one culture medium(for short: new medium) which could keep the life of Neisseria(N.) meningitidis in low temperature,and could augment the number of N.meningitidis.Methods: One culture medium containing new bovine serum and two kinds of antibiotic was prepared.The effects of keeping life of N.meningitidis were detected by known number of A and C types of N.meningitidis.Then carriers of N.meningitidis in the crowd were detected,compared with the routine detecting method as control.R...