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细胞骨架蛋白肌球蛋白轻链与癌蛋白TRE17相互作用的鉴定 被引量：2

Identification of cytoskeletal MLC2 interaction with oncogene TRE17
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摘要 目的:探讨MLC2与TRE17之间的相互作用。方法:构建了MLC2原核表达质粒,在用pGEX蛋白表达系统表达和纯化GST-MLC2和GST蛋白后,用GST Pulldwon方法进一步观察MLC2与TRE17之间的相互作用。结果:限制性内切酶分析和DNA序列测定表明MLC2 cDNA完整ORF以正确的阅读框架插入pGEX-6P-1载体;IPTG诱导表达的GST-MLC2和GST蛋白纯度在90%以上;GST Pulldown分析表明癌蛋白TRE17(447)结合GST-MLC2融合蛋白,而不是对照GST蛋白。结论:MLC2是TRE17的特异性结合蛋白。 Objective: To identify interaction of cytoskeletal MLC2 with oncogene TRE17.Methods: Plasmid GST-MLC2/pGEX was constructed in this study and used to express GST-MLC2 fusion protein.After purification of GST-MLC2 and GST proteins,MLC2 interaction with TRE17 was tested by GST pulldown assay.Results: The MLC2 cDNA was inserted into pGEX-6P-1 vector with correct open reading frame.After expression and purification of GST-MLC2 and GST proteins,GST pulldown assay suggested the association of TRE17(447) with MLC2,...
作者 杨永刚 田田 郭丹 武慧娟 王露 徐宁 沈传陆
机构地区 东南大学基础医学院
出处 《南通大学学报（医学版）》 2008年第2期82-84,共3页 Journal of Nantong University(Medical sciences)
基金 国家自然科学基金资助项目(30570696)
关键词 癌基因 肌球蛋白轻链 蛋白质相互作用 Oncogene Myosin light chain Protein interaction
分类号 R730.2 [医药卫生—肿瘤]
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参考文献10

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共引文献4

同被引文献46

  • 1沈传陆.癌基因TRE17与肌钙蛋白C2相互作用的鉴定[J].中国病理生理杂志,2005,21(12):2388-2391. 被引量：5
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南通大学学报（医学版）
2008年 第2期

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