重组葡激酶致突变检测试验

Examination of the Mutagenetic Effect of the Recombinant Staphylokinase

背景与目的 :观察重组葡激酶有否遗传毒性。材料与方法 :分别经Ames试验、CHL细胞染色体畸变试验和昆明种小鼠骨髓嗜多染红细胞微核试验对其进行了系统检测。结果 :在5个给药剂量(40、20、5.7、3.5、0.7mg/皿)和±S9 mix的情况下 ,重组葡激酶对组氨酸营养缺陷型鼠伤寒沙门氏菌株TA97、TA98、TA100 和TA102 均无诱发回变作用 ;CHL细胞经重组葡激酶3个剂量(5、2.5和1.25mg/ml)作用一定时间 ,无论在有否活化剂的情况下亦未检测到明显的细胞染色体畸变 ;昆明种雄性成熟小鼠经3个剂量(500、250、125mg/kg)的重组葡激酶尾静脉注射给药后 ,股骨骨髓微核检查结果为阴性。结论

BACKGROUND&AIM: To investigate if the recombinant staphylokinase has the effect of mutagenesis. MAˉTERIAL AND METHODS: Ames test,chromosomal aberration test in CHL cell and micronucleus test in mouse bone marrow were carried out. RESULTS: The recombinant staphylokinase at0.7～40mg/plate induced neither remarkable nor dose-dependent reverse mutagenecity to four auxotrophic mutants TA 97 ,TA 98 ,TA 100 and TA 102 with or without S 9 mix.Concentrations between1.25and5mg/ml induced neither a dose-dependent nor s...