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薯蓣皂苷元对U-2OS细胞MMP-9／TIMP-1 mRNA的影响 被引量：2

Effect of Diosgenin on mRNA of Human Osteosarcoma U-2OS Cell Line MMP-9 and TIMP-1
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摘要 为探讨薯蓣皂苷元抗肿瘤作用,采用四氮唑盐还原法(MTT法)观察薯蓣皂苷元对人骨肉瘸U-2OS 细胞体外生长的作用,运用RT-PCR方法探讨人骨肉瘤U-2OS细胞在薯蓣皂苷元作用后,其基质金属蛋白酶- 9(MMP-9)和基质金属酶组织抑制剂-1(TIMP-1)mRNA所发生的变化,结果薯蓣皂苷元对U-2OS细胞的生长具有明显的抑制作用,IC50(24h)为45 μmol／L:薯蓣皂苷元抑制U-2OS细胞MMP-9基因转录,而TIMP-1基因转录不受影响。说明薯蓣皂苷元明显抑制U-2OS细胞生长,抑制其MMP-9基因转录,对骨肉瘤侵袭和转移有一定抑制作用。 Objective: To investigate the antitumor activity of diosgenin in vitro. Methods: Cytotoxicity of diosgenin on U-2OS cell line was determined with MTT assay, its effect on the mRNA expression of matrix metalloproteinase 9 (MMP-9) and tissue inhibitors of matrix metalloproteinases 1 (TIMP-1) in U-2OS cell line was investigated by semi-quantitative RT-PCR analysis. Results: The results demonstrated that diosgenin had a significant inhibition on the U-2OS cell line and its IC50 value was 45μmol/L. RT-PCR analys...
作者 洪振强 林建华
机构地区 福建中医学院骨伤系 福建医科大学附属第一医院
出处 《福建中医学院学报》 2006年第3期21-24,共4页 Journal of Fujian College of Traditional Chinese Medicine
关键词 薯蓣皂苷元 U-2OS细胞 MMP-9/TIMP-1 MTT RT-PCR diosgenin U-2OS cell line MMP-9/TIMP-1 MTT RT-PCR
分类号 R285.5 [医药卫生—中药学]
  • 相关文献

参考文献7

  • 1王丽娟,王岩,陈声武,马吉胜,付勤,王本祥.薯蓣皂苷元体内、外的抗肿瘤作用[J].中国中药杂志,2002,27(10):777-779. 被引量：104
  • 2[2]Moalic S,Liagre B,Corbiere C,et al.A plant steroid,diosgenin,induces apoptosis,cell cycle arrest and COX activity in osteosarcoma cells[J].FEBS Lett,2001,506(3):225-230.
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  • 5陈雷,林建华,张声.CD44v6,MMP-9和TIMP-1在骨肉瘤中的表达及临床意义[J].福建医科大学学报,2000,34(4):327.
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二级参考文献10

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共引文献130

同被引文献16

引证文献2

二级引证文献2

福建中医学院学报
2006年 第3期

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