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猪圆环病毒Ⅱ型特异抗体检测间接ELISA法的建立 被引量：3

Development of indirect-ELISA method for detecting specific antibody of type Ⅱ porcine circovirus
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摘要 利用巴斯德毕赤酵母表达的猪圆环病毒2型株Cap蛋白为包被抗原,成功地建立了一种检测血清中PCV-2特异抗体的间接ELISA方法.确定了血清样品阴、阳性判定标准.临床检测结果表明,自建ELISA试剂盒具有较高的敏感性和特异性. The recombinant Cap protein of type Ⅱ porcine circovirus expressed in Pichia pastoris was used as antigen for developing an directed-ELISA assay to detect PCV-2 specific antibody.Conditions were optimized for using these preparations for an PCV-2 specific ELISA.Following the determination of conditions of ELISA,the standard value of ELISA was defied.Serum samples from porcine were detected for the antibody to PCV-2 by using the developed ELISA.It was found that the specificity and sensitivity of the develop...
作者 魏凡华 曹瑞兵 张玉颖 吴润 吴延功 张晓勇 陈溥言
机构地区 甘肃农业大学动物医学院 南京农业大学农业部疫病诊断与免疫重点开放实验室 农业部动物检疫所
出处 《甘肃农业大学学报》 CAS CSCD 2006年第6期10-13,共4页 Journal of Gansu Agricultural University
关键词 猪圆环病毒Ⅱ型 重组CAP蛋白 间接ELISA type Ⅱ porcine circovirus recombinant Cap protein indirect-ELISA
分类号 S854.43 [农业科学—临床兽医学]
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参考文献13

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二级参考文献39

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甘肃农业大学学报
2006年 第6期

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