采前苯丙噻重氮(BTH)处理对厚皮甜瓜果实防御酶及抗性物质的诱导

Defense enzymes and disease resistance substances induction in muskmelons by preharvest benzothiazole(BTH) spraying

分别于甜瓜开花前1周、幼果期(花后2周)、果实迅速膨大期(花后3周)和网纹形成期(花后4周)用100mg/L BTH对甜瓜植株进行1、2、3、4次喷洒,并分别在幼果期、膨大期、网纹期和成熟期进行取样,分析BTH处理后果实体内3种防御性酶活性和抗性物质的变化.结果表明:BTH处理能有效地提高过氧化物酶(POD)、多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)活性;增加总酚、富含羟脯氨酸糖蛋白(HRGP)及木质素含量.且随BTH处理次数的增加,3种酶活性和抗性物质的含量依次升高,其中,处理3次和4次的效果明显优于同期对照及1次和2次处理者.

The plants of muskmelon(cv.Yindi) were sprayed with benzothiazole(BTH) at 100 mg/L for 1,2,3 and 4 times at 1 week before flowering,young fruit period,fruit enlarging period and netting period,respectively.Some parameters of resistance were analyzed afterone week of treatment.The results showed that the activities of peroxidase(POD),polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL)increased significantly in treated fruit.BTH treatment also promoted the accumulation of total phenolics,hydroxyproli...