中国南方部分汉族人群HLA基因位点DRB1-DRB3、DRB1-DRB4与DRB1-DRB5单倍型分析

Analysis on HLA DRB1-DRB3、 DRB1-DRB4 and DRB1-DRB5 Haplotype Among Part Population of Han Nationality of Southern Chinese

目的初步揭示中国南方汉族人群HLA基因位点DRB1-DRB3、DRB1-DRB4与DRB1-DRB5单倍型的分布特征。方法应用聚合酶链反应-序列特异性引物法(PCR-SSP)对101例中国南方汉族人的20个HLA-DRB1及HLA-DRB3、HLA-DRB4、HLA-DRB5共23个HLA-DR等位基因进行分型。结果(1)在101例中国南方汉族人DRB1-DRB3、DRB1-DRB4与DRB1-DRB5单倍型频率分析中,DR13.3与DRB3、DRB4和DRB5具有较高的单倍型频率,DR103、DR14.3基因则基本不与DRB3、DRB4和DRB5基因连锁;(2)除少数基因位点外,大多数HLA-DRB1基因与HLA-DRB3、HLA-DRB4、HLA-DRB5基因呈现出正连锁不平衡。结论中国南方汉族人群具有自己独特的DRB1-DRB3、DRB1-DRB4与DRB1-DRB5单倍型特征。

Objective To study distribution characteristic of HLA DRB1-DRB3、 DRB1-DRB4 and DRB1-DRB5 haplotypeamong part population of Han Nationality of Southern Chinese.Method PCR-SSP technique was applied to detect HLA-DRB3, HLA-DRB4, HLA-DRB5 and 20 alleles of HLA-DRB1 gene sites of 101 people who come from Guangdong, Guangxi,Hunan, Hubei, Jiangxi and Fujian.Results The first, there was higher haplotype frequency between DR13.3 andDRB3, DRB4 and DRB5 than others while DR103 and DR14.3 did not almost link with DRB3,...