摘要
目的观察白及胶对成纤维细胞形态及活性的影响。方法分离培养日本大耳白兔胆管成纤维细胞,加入不同浓度白及胶进行共同培养,在光镜下观察日本大耳白兔胆管成纤维细胞形态的改变,用MTT法比色法检测日本大耳白兔胆管成纤维细胞活性。结果白及胶组成纤维细胞胞体较小,胞突少,多呈圆形,胞核较小。白及胶组0.1、1.0、10.0mg/mL白及胶在作用72h时成纤维细胞活性与对照组比较差异有统计学意义(P<0.01,P<0.05)。结论白及胶对日本大耳白兔胆管成纤维细胞形态的影响及白及胶对日本大耳白兔胆管成纤维细胞活性的抑制作用,可能是白及胶防治日本大耳白兔腹腔粘连的机制之一。
Objective To observe the influence of Hyacinth bletilla decocted water on fibroblastic morphology and activity. Methods Fibroblastic cells were separated from culture rabbit biliary duct.The different concentration of Hyacinta bletilla decocted water were added and cocultured.Changes of fibroblastic morphology were observed by light microscope.Fibroblastic activity were detected by MTT colorimetric method.Results In Hyacintn bletilla group fibroblastic cell hare small soma,a little spine.There was obvious d...
出处
《河北中医》
2007年第8期752-753,共2页
Hebei Journal of Traditional Chinese Medicine
关键词
成纤维细胞
白及胶
药物作用
Fibroblastic cell
Hyacinth bletilla
Drug effect
