TaMyb2-Ⅱ基因在普通小麦(Triticum aestivum L.)及其近缘种中的单核苷酸多态性分析

Single Nucleotide Polymorphism of TaMyb2-Ⅱ Gene in Common Wheat(Triticum aestivum L.)and Its Relatives

以39份抗旱性不同的普通小麦、5份A基因组材料、4份拟斯卑尔脱山羊草(Aegilops speltoides)、6份粗山羊草(A.tauschii)和2份四倍体小麦为材料,分析TaMyb2基因的核苷酸序列长度多态性和单核苷酸多态性,及其与抗旱性的关系。结果发现,TaMyb2在A基因组材料中无目标片段扩增,在其他材料中共检测到Ⅰ、Ⅱ、Ⅲ3种类型序列。经详细分析,TaMyb2-Ⅱ序列长1606bp,在供试材料77088bp的核苷酸序列中检测到34个单核苷酸变异,其中26个SNP,8个InDel,二者出现的频率分别为1/2965bp和1/9636bp,编码区π值(0.00055)小于非编码区的π值(0.00185),说明编码区的遗传变异小于非编码区的遗传变异。从SNP水平上分析,发现普通小麦与其D基因组供体种粗山羊草及四倍体小麦的亲缘关系较近,与B基因组供体种拟斯卑尔脱山羊草的亲缘关系较远。48份材料的TaMyb2-Ⅱ序列共分为18个单倍型(haplotype),其中haplotype2、3、5、6、8、9均为旱地栽培的普通小麦品种,说明普通小麦TaMyb2-Ⅱ的这几个haplotype结构可能与抗旱性有关。

Drought is one of the major elements affecting the yield of wheat,one of the most important crops in arid and semi-arid areas.Mining pivotal drought-resistant genes and probing their polymorphisms is a feasible approach to decipher crops drought resistance.The genes involved in drought resistance are classified into two classifications,functional genes and regulatory genes,based on their functions responding to water deficient.Much attention has been paid to regulatory genes because of their crucial roles ...