细胞松弛素B对日本沼虾四倍体的适宜诱导条件的研究

STUDY ON INDUCEMENT CONDITION OF TETRAPLOID IN MACROBRACHIUM NIPPONENSE BY CYTOCHALASIN B

本文研究了用细胞松弛素B(Cytochalasin B,CB)诱导日本沼虾(Macrobrachium nipponense)多倍体的处理条件和方法。试验水温分别为18—19℃、22—23℃,CB浓度为1.0—2.0mg/L,处理的起始时间在受精后5—9h,处理的持续时间为10—20min。结果表明,在水温为18—19℃时,四倍体卵子的最适诱导条件为:受精后7—8h,CB溶液的浓度1.5mg/L,处理20min,四倍体诱导率最高可达56.1%;在水温为22—23℃时,最适诱导条件为:在受精后6 h左右,CB溶液浓度为1.5mg/L,处理15—20min,四倍体诱导率最高可达54.3%。处理的起始时间和CB溶液的浓度对胚胎的四倍体率有显著影响(P<0.05);胚胎的成活率随CB浓度的提高与处理的持续时间的延长而下降。考虑到胚胎成活率因素,建议选择CB浓度为1.5mg/L,处理持续时间15—20min。

Cytochalasin B(CB) was used for inhibiting the first cleavage of zygotes to induce tetraploid in Macrobrachium nipponense. At 18—19℃ and 22—23℃ water temperature,two tetraploid induction trials designed by L_9(3~4) were carried out using CB treatment at different beginning time after fertilization(5 hours,6 hours,7 hours,8 hours,9 hours),different concentration of CB(1.0mg/L,1.5mg/L,2.0mg/L),and different treatment duration time of the eggs(10 min,15 min,20 min).The results indicated that the highest induct...