摘要
本研究以酪蛋白分解物为蛋白源配制三种微粒子饲料MD-S、MD-T和MD-U对真鲷开口仔鱼进行饲养试验。以MD-S的配方为基准,MD-T采用粉状大豆卵磷脂和麸质代替液状大豆卵磷脂;MD-U则另外添加0.1%的肽酶。结果表明,微粒子饲料在水中浸泡15min后,MD-T的溶出率(35.5%)低于MD-S(46.8%)和MD-U(45.8%);实验结束时(20日龄),仔鱼的成活率以生物饵料(轮虫)组为最高(86.3%),其次是MD-T组为20.7%,显著高于(P<0.05)MD-S组(13.3%)和MD-U组(13.6%);生物饵料组的仔鱼全长(6.14±0.49mm)显著大于微粒子饲料组(4.23±0.30mm^4.46±0.30mm),各微粒子饲料组之间仔鱼的全长并不存在显著差异(P>0.05)。在孵化后第12d,微粒子饲料组的仔鱼肠上皮细胞发育良好,但至孵化后第18d,仔鱼肠上皮细胞大部分萎缩、并发生脱落。鱼体的蛋白质、DNA与RNA日间增长率微粒子饲料MD-T组高于MD-S和MD-U组,但都低于生物饵料组。由此可见,微粒子饲料中添加肽酶并无助真鲷仔鱼对其消化吸收;可是,使用粉状大豆卵磷脂与麸质代替液状卵磷脂能增强微粒子饲料的黏合性,可减少其营养成分的溶出率,从而提高微粒子饲料的饲育效果。
This study investigates the effects of exogenous enzyme supplementation on the digestibility of microparticle diets (MD). Powder soybean lecithin and gluten were used instead of paste soybean lecithin for evaluating its effect on the leaching rate in MD. Three kinds of MD (MD-T; MD-S; MD-U) were prepared. Based on formulation of MD-S,MD-T included powder soybean lecithin instead of paste soybean lecithin and gluten as a binder. MD-U was supplied with 0.1% of peptidase. The other ingredients were the same in...
出处
《水生生物学报》
CAS
CSCD
北大核心
2006年第6期734-741,共8页
Acta Hydrobiologica Sinica
基金
The project sponsored by Taiyo Yushi Co.,Ltd.Yokohama ,Japan
关键词
真鲷仔鱼
微粒子饲料
肽酶
大豆卵磷脂
Pagrus major larvae
Microparticle diet
Peptidase
Soybean lecithin
