摘要
以散叶生菜大速生(Lactuca sativavar.capatataL.)为试材,以MS为基本培养基,采用不同激素配比,确定生菜高效诱芽培养基为MS+1.5mg/L6-BA+0.2mg/LIAA;抗生素敏感性试验表明,筛选培养基中适宜的潮霉素选择压为20mg/L,抑菌剂羧苄青霉素的适宜质量浓度为300mg/L;通过根癌农杆菌介导的叶盘法将携带O型和A型口蹄疫抗原决定簇融合基因O21-O14-A21-HBcAg转入大速生散叶生菜,对部分抗性植株进行PCR和PCR-Southern杂交检测,证实目的基因已经成功整合到生菜基因组中。RT-PCR检测初步表明,O21-O14-A21-HBcAg基因可以在生菜中正常转录表达。
In this experiment,the effects of different hormone concentration on shoot regeneration from cotyledon explants are investigated.The results show that MS medium supplied with 1.5 mg/L 6-BA and 0.2 mg/L IAA is the suitable shoot regeneration.The experiment on sensitivity of cotyledon to hygromycin B and carbenicillin shows that the suitable hygromycin concentration for selecting transgenic tissue is 20 mg/L and the carbenicillin concentration is 300 mg/L.Fused gene O21-O14-A21 containing both type O and A FM...
出处
《云南植物研究》
CSCD
北大核心
2007年第1期98-102,共5页
Acta Botanica Yunnanica
基金
福建省科技计划项目(JY-03-B-30-20)
关键词
口蹄疫抗原决定簇
根癌农杆菌
生菜
遗传转化
基因表达
FMD epitopes
Agrobacterium tumefaciens
Lactuta sativa
Genetic transformation
Gene expression
