SLE患者外周血γδT细胞系的建立及表型和功能的研究

Expansion of γδT cells from SLE patients and analysis of their phenotypes and functions

目的:建立体外扩增系统性红斑狼疮(SLE)患者外周血γδT细胞的方法,并初步分析其表型和功能,探索γδT细胞在SLE发病中的作用。方法:采用单克隆抗体固相法,对15例SLE患者和8例正常人外周血γδT细胞进行体外扩增建系,以流式细胞仪检测γδT细胞表型,并用MTT法观察γδT细胞对Daud i细胞的细胞毒作用。结果:建立了SLE患者外周血γδT细胞系,其平均纯度为(58.1±11.2)%,较正常对照组(80.3±9.2)%偏低(P<0.05);其细胞表型为:Vδ1(34.4±24.5)%、Vδ2(61.9±28.6)%、Vδ3(16.1±10.6)%、Vγ9(76.4±11.8)%,其中Vδ2表达较对照组降低,而Vδ1和Vδ3表达增加(P均<0.05);其细胞毒作用在二组间无明显差别。结论:SLE患者外周血γδT细胞的Vδ、Vγ基因的取用表达存在差异,提示其在SLE的发病中可能起一定作用。

Aim: To expand the γδT cells from SLE patients and analyze their phenotypes and functions to explore their roles in the pathogenesis of SLE.Methods: The PBMCs from 15 SLE patients were cultured with solid phase anti-TCRγδ antibody,phenotypes were then analyzed with flow cytometric assay and the cytotoxic activity of γδT cells against Daudi cells was determined using MTT calorimetric assay.Results: SLE γδT cell strains were established and their average purification was(58.1±11.2)%,less than normal controls(...